Journal: PLOS One

Article Title: Evidence of an allostatic response by intestinal tissues following induction of joint inflammation

doi: 10.1371/journal.pone.0338053

Figure Lengend Snippet: Adjacent FFPE-sections of Swiss-rolled ileum or colon stained with Alcian Blue ( A ) or the biotinylated GSI lectin ( B ) as described in the Methods. Brown = positive stain; Black arrows show the increased mucin secretion with enhanced GSI staining in the DI at 3d post IAI of CFA. Modified gene transcripts in the mucin biosynthesis pathways in proximal and distal regions of the ileum and the distal colon, which were affected by IAI of CFA are summarized in the heat map in panel ( C) . PI = Proximal Ileum, DI = Distal Ileum, PC = Proximal Colon, DC = Distal Colon.

Article Snippet: Sections of intestinal Swiss rolls were stained using the Alcian Blue Kit (Vector Labs, H-3501), anti-CD68 (1:100), anti-CD4 (1:2000), anti-CD8 (Abcam, Ab237709 , 1:500) or anti-Ki67 (Invitrogen, MA5–14520; 1:100), Invitrogen, MA5–14520) or the following biotinylated lectins (all from Vector Labs), Griffonia Bandeiraea Simplidifolia Lectin I, (GSL I, B-1105–2), Maackia Amurensis Lectin II (MAL II, B-1265–1) and Ulex Europaeus Agglutinin I (UEA, B-1065–2).

Techniques: Staining, Modification